Fig. 4.
Requirement for Gpr124 and Reck function in limb development. (A) Alcian Blue staining of the skeleton at E15 showing progressively more severe truncations with progressively reduced Gpr124 and/or Reck function (from left to right). Red arrow points to a region with local bleeding (see Fig. S1 for additional examples). (B) Quantification of E15 limb skeletal defects with progressively reduced Wnt7a and/or Gpr124 function. Δ, ReckΔex2; PW, ReckPW. The scoring procedure is described in the Materials and Methods section. P-values were calculated with the non-parametric Mann–Whitney–Wilcoxon test. Error bars show the mean±s.d. Each circle represents a single limb.