Fig. 6.

Synergy between loss of Tbx3 and loss of Wnt7a in the severity of limb development phenotypes. (A) Alcian Blue staining of the skeleton at E15 showing progressively more severe truncations with progressively reduced Tbx3 in a Wnt7a^−/− background or with early embryonic loss of Tbx3. (B) Quantification of E15 limb skeletal defects with progressively reduced Wnt7a and/or Tbx3 function. The scoring procedure is described in the Materials and Methods section. P-values were calculated with the non-parametric Mann–Whitney–Wilcoxon test. Error bars show the mean±s.d. Each circle represents a single limb.