TABLE 2.

Influence of LY29 and DIC on proteinase and peptidase activities of mixed ruminal microorganisms and on bacteriolytic activity of mixed ruminal protozoa

Substrate	Rate of breakdowna
	Control	DIC (μM)		LY29 (μM)		SE
		2	10	2	10
Proteinase activity (mg of 14C-casein hydrolyzed h−1 mg of protein−1)	0.124	0.120	0.122	0.124	0.124	0.002
Peptidase activity (nmol min−1 mg of protein−1)
GlyArg-pNA	1.06	1.02	1.13	1.03	1.04	0.041
GlyPro-pNA	2.57 C	2.42 A	2.35 A	2.45 B	2.34 A	0.041
Ala2-pNA	5.78	5.57	5.64	5.73	5.47	0.098
Leu-pNA	0.19	0.21	0.24	0.20	0.18	0.024
Pro-pNA	0.96	0.98	0.96	1.02	0.99	0.043
Bacteriolytic activity of protozoa (% breakdown of S. ruminantium h−1)b	6.62	6.23	5.99	6.28	6.41	0.36

^aResults are means obtained with samples from four sheep on a mixed grass hay-concentrate diet. Means followed by different letters are significantly different (P < 0.05). 

^bBacteriolytic activity was measured in only two of the sheep.