Table 1.

Hantavirus nRT–PCR (L gene) and serology results for the 21 pet rats (Rattus norvegicus) in the Cherwell Colony

APHA ID	Colour	Gender	Hantavirus nRT-PCR		PCR status	SEOV ELISA (adjusted OD)	SEOV FRNT	Serology status	PM observations/notes
			Lunga	Kidneya
3776	Brown	Female	Neg (0/4)	Neg (0/4)	Neg	0·396	1 : 160	Pos	Healthy
3777	Brown	Female	Pos (2/3)	Pos	Pos	0·2	1 : 2560	Pos	Healthy
3778	Brown	Female	Pos (1/4)	Neg (0/4)	Pos	No blood	No blood	nd	Found dead. Vaginal discharge, nose bleed
3779	Brown	Female	Neg (0/4)	Pos (4/4)	Pos	0·517	1 : 160	Pos	Fatty liver
3780	Brown	Female	Pos	Pos	Pos	nd	⩾1 : 40	Pos	Healthy
3781	Brown/white	Female	Pos	Pos	Pos	0·79	>1 : 10 240	Pos	Healthy
3782	Brown/white	Female	Pos	Pos	Pos	0·643	>1 : 10 240	Pos	Healthy
3783	Brown	Female	Neg (0/4)	Pos (4/4)	Pos	0·76	1 : 640	Pos	Healthy
3784	Brown	Male	Pos	Pos	Pos	1·05	1 : 10 240	Pos	Enlarged pale kidneys. Imported to UK in 2011.
3785	Brown	Female	Pos	Pos	Pos	0·606	1 : 2560	Pos	Healthy
3786	Black/white	Female	Neg (0/4)	Neg (0/4)	Neg	0·686	>1 : 2560	Pos	Healthy
3787	Brown	Male	Pos	Pos	Pos	0·09	1 : 640	Pos	Healthy
3788	White	Female	Neg (0/4)	Neg (0/4)	Neg	0·245	1 : 640	Pos	Healthy
3789	Brown	Female	Pos	Pos	Pos	0·96	1 : 2560	Pos	1·5 cm tumour, hard mass in right mammary gland
3790	Brown	Male	Pos	Pos	Pos	0·676	1 : 640	Pos	Healthy
3791	Golden	Male	Neg (0/4)	Pos	Pos	0·89	1 : 2560	Pos	Healthy
3792	Dark brown	Female	Pos	Pos	Pos	0·63	>1 : 10 240	Pos	Healthy
3793	Pale Brown	Male	Pos (1/3)	Pos	Pos	0·33	1 : 2560	Pos	Healthy
3794	White	Female	Neg (0/4)	Neg (0/4)	Neg	0·875	1 : 160	Pos	Healthy
3795	Mix colour	Male	Pos	Pos	Pos	0·633	>1 : 10 240	Pos	Healthy
3796	Brown/white	Female	Pos (4/4)	Neg (0/4)	Pos	0·461	1 : 640	Pos	Healthy
Total positive			14/21	15/21	17/21			20/20

OD, optical density; Pos., positive; Neg., negative; nd, not determined.

^aUnless otherwise stated, the reported RT–PCR results were observed in triplicate. Positive controls: D+VE RAT (ELISA 1·0 and FRNT 1 : 2560) and KAP KIN (ELISA 0·624 and FRNT 1 : 640).