. 2017 Jun 15;145(11):2212–2220. doi: 10.1017/S0950268817001182

Table 2.

Sequence of PCR primers used in this study

ORFs	Primer sequence (5′−3′)	Product size (bp)	Reference
ctxB Fw-con	ACTATCTTCAGCATATGCACATGG	186	Morita et al. [13]
ctxB Rv-cla	CCTGGTACTTCTACTTGAAACG
ctxB Rv-elt	CCTGGTACTTCTACTTGAAACA
ctxA F	CTCAGACGGGATTTGTTAGGCACG	302	Keasler et al. (1993)
ctxA R	TCTATCTCTGTAGCCCCTATTACG	302
tcpA^cla F	CACGATAAGAAAACCGGTCAAGAG	618
tcpA^claR	ACCAAATGCAACGCCGAATGGAG	618
tcpA^elt F	GAAGAAGTTTGTAAAAGAAGAACAC	472
tcpA^elt R	GAAAGGACCTTCTTTCACGTTG	472
toxR F	CCTTCTGATCCCCTAAGCAATAC	779	Singh et al. (2001)
toxR R	AGGGTTAGCAACGATGCGTAAG	779	Singh et al. (2001)
zot F	TCGCTTAACGATGGCGCGTTTT	947	Singh et al. (2001)
zot R	AACCCCGTTTCACTTCTACCCA	947	Singh et al. (2001)
rtxC F	CGACGAAGATCATTGACGAC	265	Chow et al. (2001)
rtxC R	CATCGTCGTTATGTGGTTGC	265	Chow et al. (2001)
rtxA F	CTGAATATGAGTGGTTGACTTACG	418	Chow et al. (2001)
rtxA R	GTGTATTGTTCGATATCCGCTACG	418	Chow et al. (2001)
rtxA1	GCGATTCTCAAAGAGATGC	1366	Lin et al. (1999)
rtxA2	CACTCATTCCGATAACCAC	1366	Lin et al. (1999)
tlc3	GGGAATGTTGAGTTCTCAGTG	1548	Rubin et al. (1998)
tlc4	GTTGCGAAGTGGATTTTGTG	1548	Rubin et al. (1998)
intl4 : 3	CCTTCATTGGATCACTCG	597	O'Shea et al. [12]
intl4 : 4	GACGGAAAAAGATAGTGCC	597	O'Shea et al. [12]
acfB1	GATGAAAGAACAGGAGAGA	1000	O'Shea et al. (2002)
acfB2	CAGCAACCACAGCAAAACC	1000	O'Shea et al. (2002)
orfUF	CGTCACACCAGTTACTTTTCG	1072	O'Shea et al. (2002)
orfU R	AGAATGTACGCCATCGC	1072	O'Shea et al. (2002)
rstC1	AACAGCTACGGGCTTATTC	238	Waldor et al. (1997)
rstC2	TGAGTTGCGGATTTAGGC	238	Waldor et al. (1997)
rstC F	ATGAGTTTGAAACCATACACTTT	238	Waldor et al. (1997)
rstC R	TTACAGTGATGGACTCAGTCAAT	238	Waldor et al. (1997)
rstA1	ACTCGATACAAACGCTTCTC	1009	O'Shea et al. (2002)
rstA2	AGAATCTGGAAGGTTGAGTG	1009	O'Shea et al. (2002)
tcpI F	TAGCCTTAGTTCTCAGCAGGCA	862	Rivera et al. (2001)
tcpI R	GGCAATAGTGTCGAGCTCGTTA	862	Rivera et al. (2001)
rstR₁(forward)	CTTCTCATCAGCAAAGCCTCCATC	500	Nusrin et al. [21]
rstR₂(forward)	GCACCATGATTTAAGATGCTC
rstA3(reverse)	TCGAGTTGTAATTCATCAAGAGTG
VSP-I
VCO183F	CAGTAAGAGTGTAGCGTGCC	950	O'Shea et al. [12]
VCO183R	CCTGCACATCGAGATGC	950	O'Shea et al. [12]
VSP-II
VC0511F1	CTTGCTGCGTACTTAGCA	385	Nusrin et al. (2009)
VC0511R1	AGTAGCATCGCTCTCGTA	385	Nusrin et al. (2009)
VC0513F1	CTGAGGTGTTATATGTTTCG	781	Nusrin et al. (2009)
VC0513R1	TCAAATTTCCTGACAGTTCC		Nusrin et al. (2009)
VC0514F1	GTTTGGGAAGGGTACACA	1·651	Nusrin et al. (2009)
VC0514R1	GCTCTTCAGCCGCTGA	1·651	Nusrin et al. (2009)
VC0515F1	GGTGGTGCTGCATGGA	1·135	Nusrin et al. (2009)
VC0515R1	TCTAAAGCCTCACACCA	1·135	Nusrin et al. (2009)
VCO516F	GTTTTCTGCGTTGTTCGAG	950	O'Shea et al. [12]
VCO516R	TCCTGATGTCTCTCTTGCCG	950	O'Shea et al. [12]