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. 2022 May 29;12:8989. doi: 10.1038/s41598-022-12961-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Chemerin system expression in embryo annexes and fluids. (A) Protein abundance of chemerin detected by western blotting within the albumen (thin and thick), allantoic membrane, amniotic membrane, and the perivitelline membrane from fertilised eggs incubated for 7 days (n = 6). The ratio chemerin/ponceau is represented. (B) Localisation of the different embryonic structures at ED7. (C–E) Protein abundance of chemerin (C), Chemokine-like receptor 1 (CMKLR1) (D), G Protein-coupled Receptor 1 (GPR1) (E) and Chemokine (C–C motif) receptor-like 2 (CCRL2) (F) detected by western blotting within the allantoic membrane of incubated eggs at different embryonic days (ED): 7, 9, 11, 14, 16, and 18 (n = 8 per stage). Values are expressed as mean ± standard errors of means. Letters indicate significant differences between various conditions (p < 0.05).