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. 2022 May 29;12:8989. doi: 10.1038/s41598-022-12961-4

Table 1.

List of primers used for qRT-PCR.

Gene Primer forward Primer reverse Efficiency
RARRES2 CGCGTGGTGAAGGATGTG CGACTGCTCCCTAAAGAGGAACT 1.90
CMKLR1 CGGTCAACGCCATTTGGT GGGTAGGAAGATGTTGAAGGAA 1.95
GPR1 ACCTGCCTGAGGAAGAAGAA AAAGGCCAGTGGAAGCCCAT 2.00
CCRL2 CACGCAGTGTTTGCTTTAAAAGC CAACAGCCCACGTGACAATG 1.92
CALD1 TTGAGCGTCGCAGAGAACTT AGGCGTTTTTGGCGTCTTTC 2.04
TEM8 TGAGAGGGAGGCCAATCGGTCA GCAGCGGCCCTTGTCTCCTG 2.00
EPHRIN B2 TTCCCCACAACACACCACAA TCGCCGCTGACCTTTTCATA 1.95
CXCR4 TGTGTATGTGGGTGTCTGGC CAGCCAGTTGTCATGAGGGT 1.94
TIE2 CTGAGTGCTGGGATGCTACC GTGATAGACTCACGGGAGCG 2.00
GAPDH TGCTGCCCAGAACATCATCC ATCAGCAGCAGCCTTCACTACC 1.98
EEF1a AGCAGACTTTGTGACCTTGCC TGACATGAGACAGACGGTTGC 1.95
Β Actin ACGGAACCACAGTTTATCATC GTCCCAGTCTTCAACTATACC 2.02