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The formation of a single lumen. (A,B) F-actin was concentrated at the apical area in the neural rosette. (C,D) ZO-1 was concentrated at the apical area in the neural rosette. (E) F-actin co-localized with ZO-1 at the apical area. (F) The fluorescence intensity of F-actin and ZO-1. Scale bars: 50 μm. *P < 0.05.
