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. 2022 May 16;13:893576. doi: 10.3389/fimmu.2022.893576

Figure 2.

Figure 2

Phenotype and functionality of thyTreg cell product. (A) Frequency of phenotypic and functionality markers within thyTreg cells (day 7). (B) Frequency of homing markers within thyTreg cells (day 7). (C) Quantitation of molecules secreted in day 7 thyTreg culture supernatants. Anti-inflammatory molecules in blue; proinflammatory molecules in red. (D) Representative flow cytometry histograms showing CD4 (green) and CD8 T (purple) cell proliferation as CellTrace Violet lost. C-, negative control of proliferation, PBMC cultured alone without stimulation; C+, positive control of proliferation, PBMC cultured alone with anti-CD3/anti-CD28 stimulation; 1:1 to 1:8, stimulated PBMC cultured with thyTreg cells at different thyTreg : PBMC ratios. (E) Summary of the suppressive capacity of thyTreg cells defined as % inhibition of CD4 (green) and CD8 T (purple) cell proliferation at the indicated ratios. Graphs show mean ± SEM.