FIG. 2.

Detection of PhaC1 polymerase by Western blot analysis of E. coli 193MC1. Western blotting was performed with polyclonal antibodies raised against PhaC1 of P. oleovorans. Cells of E. coli 193MC1 were cultured in E2 minimal medium in the absence (lane 1) and in the presence (lane 2) of 1 mM inducer (3-MB). Lane 3 contained purified PhaC1 polymerase. All lanes contained 25 μg of protein. The molecular masses (in kilodaltons) of the marker proteins and the position of the PhaC1 polymerase are indicated on the left and right, respectively.