TABLE 1.
Bacterial strains and plasmids and their relevant genotypes and phenotypes
| Strain or plasmid | Relevant genotype or phenotypea | Source or reference |
|---|---|---|
| Strains | ||
| P. oleovorans | ||
| GPo1 | OCT, PHA+ | 36 |
| POMC1 | GPo1 derivative, xylS/Pm::phaC1, Kmr | This study |
| P. putida | ||
| GPp104 | NTG mutant of P. putida KT2442 (2), PHA− | 17 |
| GPMC1 | GPp104 derivative, xylS/Pm::phaC1, Kmr | This study |
| E. coli | ||
| DH10B | Host for E. coli plasmids | 11 |
| CC1118λpir | Host for pUT-derived plasmids | 13 |
| JMU193 | fadR::Tn10 fadB64, Tcr | 33 |
| 193MC1 | JMU193 derivative, xylS/Pm::phaC1, Kmr Tcr | This study |
| Plasmids | ||
| pPSW213 | RK2 derivative, Tcr, lacIq/Plac | 4 |
| pUC18Not | pUC18 derivative, Apr, NotI-flanking MCS | 13 |
| pCNB1 | mini-Tn5 delivery plasmid, Kmr Apr, xylS/Pm in mobile segment | 6 |
| pPO1 | pSW213 derivative, Tcr, lacIq/Plac::phaC1 | This study |
| pPO11 | pUC18Not derivative, Apr, 1.8-kb NotI cassette containing phaC1 | This study |
| pMC1 | pCNB1 derivative, Apr Kmr, xylS/Pm::phaC1 | This study |
a
OCT, natural plasmid enabling growth on C6 to C12 alkanes; NTG, N′-methyl-N′-nitro-N′-nitrosoguanidine; MCS, multiple cloning site.