TABLE 3.
Relative monomer compositions of isolated PHA produced by recombinant strains
Strain | Culture | 3-MB concn (mM) | CDW (g · liter−1) | Amt of PHA (% of CDW) | Monomer composition (mol%)a
|
||
---|---|---|---|---|---|---|---|
C6 | C8 | C10 | |||||
E. coli 193MC1b | Fed batch fermentation | 0.25 | 3.48 | 11 | 13 | 66 | 21 |
1.00 | 3.55 | 12 | 14 | 66 | 20 | ||
P. oleovorans POMC1c | Continuous fermentation | 0.00 | 1.50 | 43 | 10 | 89 | <1 |
0.10 | 1.67 | 53 | 13 | 86 | <1 | ||
P. oleovorans GPo1d | 10 | 89 | <1 |
Monomer compositions are expressed as molar percentages. C6, 3-hydroxyhexanoate; C8, 3-hydroxyoctanoate; C10, 3-hydroxydecanoate.
Polymers produced by E. coli 193MC1 were isolated from the culture described in the legend to Fig. 3.
Polymers produced by P. oleovorans POMC1 were isolated from the continuous culture (Table 2).
Relative monomer composition of PHA lab stock produced by wild-type P. oleovorans GPo1. Each value is the average of values from three different assays.