TABLE 1.

Degenerate primers for antibody light and heavy chains.

Chain region	Primer sequences
VH(5′–3′)	GATGTGAAGCTTCAGGAGTC CAGGTGCAGCTGAAGGAGTC CAGGTGCAGCTGAAGCAGTC CAGGTTACTCTGAAAGAGTC GAGGTCCAGCTGCAACAATCT GAGGTCCAGCTGCAGCAGTC CAGGTCCAACTGCAGCAGCCT
VH (3′–5′)	TGCAGAGACAGTGACCAGAGT TGAGGAGACTGTGAGAGTGGT TGAGGAGACGGTGACTGAGGT TGAGGAGACGGTGACCGTGGT
VL(5′–3′)-Kappa	GATGTTTTGATGACCCAAACT GATATTGTGATGACGCAGGCT GATATTGTGATAACCCAG GACATTGTGCTGACCCAATCT GACATTGTGATGACCCAGTCT GATATTGTGCTAACTCAGTCT GATATCCAGATGACTCAGTCT GACATCCAGCTGACTCAGTCT CAAATTGTTCTCACCCAGTCT
VL(3′–5′)-Kappa	CCGTTTCAGCTCCAGCTTG CCGTTTTATTTCCAGCTTGGT CCGTTTTATTTCCAACTTTG GGATACAGTTGGTGCAGCATC