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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: Mol Microbiol. 2022 Mar 4;117(5):1023–1047. doi: 10.1111/mmi.14887

Figure 7. Purified UppH breaks down UPP in A. tumefaciens.

Figure 7.

Purified His6-UppH and His6-UppH** at 1 mM were incubated for 1 h with A. tumefaciens ΔpruA CDGS-. (A) Cells were pre-attached to glass coverslips prior to incubation with UppH preparations, and subsequently labeled with af-WGA and fl-DBA. Fluorescence microscopy was performed as described in experimental procedures with only the merged phase contrast and fluorescence images shown. (B) TEM performed on cells following incubation with UppH preparations on a JEOL JEM-1010 Transmission Electron Microscope at 80 kV.