Figure 8. Two Wzy-type polymerase proteins are redundant for attachment and biofilm formation.

(A) Genetic loci of uppY and uppW. Stalked circles represent independent, sequenced transposon insertion sites and numbers with X indicate 14 or more independent transposon insertions. (B) ATGN CR colony phenotypes of the indicated deletion mutants in the ΔpruA CDGS− background. (C) Quantitative biofilm as described in experimental procedures with the ratio of A₆₀₀ of acetic acid-solubilized CV-stained coverslips to planktonic OD₆₀₀ normalized to percent wild type for the indicated in-frame deletion mutants in otherwise wild type C58 after 48 h incubation in ATGN. The indicated strains with solid black and light orange bars have the pSRK-Gm vector control, whereas the strains represented by dark orange bars harbor a P_lac-uppY plasmid and the strains with cross hatched bars harbor the P_lac-uppW plasmid. Cultures grown in 400 μM IPTG to induce P_lac. Error bars are standard deviation of assays performed in triplicate.