Skip to main content
. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: Mol Microbiol. 2022 Mar 4;117(5):1023–1047. doi: 10.1111/mmi.14887

Figure 9. Dual lectin binding of UPP with WGA and DBA.

Figure 9.

Short term attachment assay of A. tumefaciens ΔpruA (CDGS−) on a glass coverslip co-incubated with lectins. (A) UPP stained with af-WGA showing red fluorescent polar foci. (B) UPP stained with fl-DBA showing green fluorescent polar foci. (C) UPP stained with both af-WGA and fl-DBA showing the sectored pattern of dual red and green fluorescence. Arrows indicate clearly labeled UPP. Insets – magnification of boxed area. (D) Image of two attached dual labeled cells at the focal plane of surface contact. Microscopy was performed as described in experimental procedures on a Nikon Eclipse Ti-E microscope with a Hamamatsu ORCAFlash 4.0 camera with the 60X oil immersion objective.