TABLE 1.
PCR and sequencing primers
| Primer | Position | Orientation | Specificity | Sequence |
|---|---|---|---|---|
| PCR primers | ||||
| F24 | 9-27 | Forward | Universal | AGTTTGATYMTGGCTCAG |
| F25 | 1525-1541 | Reverse | Universal | AAGGAGGTGWTCCARCC |
| Sequencing primers | ||||
| C75 | 7-27 | Forward | Universal | GAGAGTTTGATYCTGGCTCAG |
| B34 | 344-358 | Forward | Universal | ACGGGAGGCAGCAGY |
| F16 | 789-806 | Forward | Universal | TAGATACCCYGGTAGTCC |
| F18 | 1099-1113 | Forward | Most bacteria | GCAACGAGCGCAACC |
| F19 | 1099-1114 | Forward | Bacteroides | ATAACGAGCGCAACCC |
| E94 | 1522-1541 | Reverse | Universal | GAAGGAGGTGWTCCARCCGCA |
| F20 | 1226-1242 | Reverse | Most bacteria | CCATTGTARCACGTGTG |
| F21 | 1226-1242 | Reverse | β-Proteobacteriaa | CCATTGTATGACGTGTG |
| F17 | 907-926 | Reverse | Universal | CCGTCWATTCMTTTGAGTTT |
| F15 | 519-533 | Reverse | Universal | TTACCGCGGCTGCTG |
| F22 | 344-358 | Reverse | Universal | RCTGCTGCCTCCCGT |
a
Members of the β subclass of the class Proteobacteria.