FIGURE 3.

Differentially regulated Transcriptional Start Sites (TSRs) in naïve versus cocaine or oxycodone exposed rat brains. (A) Heatmap of transcription initiation levels from differential TSRs in PFC naïve, oxycodone- and cocaine-exposed rats based on mean-centered log2 ratios; each row shows the closest gene and the TSR position relative to that gene’s annotated TSS. (B) Barplot of significant logistic regression MEIRLOP coefficients for top-ranked motifs associated with regulated TSRs between naïve and oxycodone or cocaine conditions in PFC and NAc. (C) Example of regulation at the Fkbp5 gene locus, including (top to bottom) Hi-C contact matrix with TAD positions, genome browser tracks showing regulated TSRs (csRNA-seq), GR binding (ChIP-seq), chromatin accessibility (ATAC-seq), and GRE motif location. Ihskb = interactions per hundred square kilobases per billion mapped reads.