Fig. 3.

Single-cell RNA-seq identifies cell populations within infection-driven MDSCs covering the full spectrum of granulocyte differentiation. a UMAP embedding of 1,687 single-cell transcriptomes from the cell clusters classical monocyte, immature myeloid cells, and neutrophils extracted from the combined dataset shown in Figure 2b. b UMAP embedding (as a) showing clusters of cells annotated by cell type. c Violin plots showing the expression of selected marker genes of the different cell clusters depicted in b. Expression in each cell population is shown along with the probability density of gene expression symbolized by the shape of the plot. d–h UMAP embeddings (as a) showing the normalized expression of Prtn3 (d), Ltf (e), Ly6g (f), Ccl6 (g), and Cebpe (h) genes. i UMAP embedding showing the cell cycle phase. UMAP, Uniform Manifold Approximation and Projection.