Fig. 6.

Infection-driven MDSCs are reliant on glucose availability for terminal differentiation. a Percentage of weight loss of S. aureus-infected mice during the course of infection. b Glucose levels in the blood of S. aureus-infected mice during the course of infection. c Representative flow cytometry contour plots showing the expression of CCL6 within Ly6G⁺ cells isolated from the spleen of S. aureus-infected mice at day 21 of infection and cultured in vitro either in the presence or absence of glucose or after inhibition of glycolysis by addition of 2-DG. d Percentage of CCL6⁺ cells within the Ly6G⁺ cell population at progressive times during in vitro culture either in the presence (black bars) or absence (white bars) of glucose or after inhibition of glycolysis with 2-DG (gray bars). Results are presented as the mean ± SD of biological replicates (N = 5 for a, b and N = 3 for c, d) and are representative of 3 independent experiments. Statistical significance: ***p < 0.001. 2-DG, 2-deoxy-D-glucose.