TABLE 2.

Release of C-terminal amino acids from bradykinin (5 nmol) and β-amyloid (5.25 nmol) by native A. oryzae carboxypeptidase I^a

Substrate	Released amino acid	Amt of released amino acid (nmol) after incubation forb:
		0 h	0.5 h	1 h	3 h	6 h	24 h
Bradykininc	Arg	0.78	4.92	4.89	4.94	4.92	5.00
	Phe	0	1.29	2.39	4.99	6.80	9.60
	Pro	0	1.05	2.09	4.21	4.59	4.91
	Ser	0	1.00	2.04	4.12	4.45	4.95
	Gly	0	0	0	0	0	3.65
β-Amyloidd	Glu	0.38	4.94	5.11	5.12	5.145	5.80
	Tyr	0	2.24	4.02	5.09	5.075	5.25
	Gly	0	2.13	3.96	5.10	5.11	5.23
	Ser	0	1.68	3.76	5.10	5.09	5.24
	Asp	0	0.10	0.50	2.93	4.47	5.23
	His	0	NAe	NA	NA	NA	2.11
	Arg	0	0	0	0	0.61	2.09
	Phe	0	0	0	0	0.47	2.17

^aThe reaction conditions are described in the text. 

^bMeans based on three measurements. A typical error in a concentration determination did not exceed 5%. 

^cThe sequence of bradykinin is Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg. 

^dThe sequence of β-amyloid is Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu. 

^eNA, the values for histidine could not be determined precisely due to the presence of a coeluting peak.