Figure 1.

ELISA performed to determine κ versus λ light-chain expression of different recombinant human IgMs obtained after transient transfection in Expi293 cells. Heavy-chain, light-chain (κ or λ), and J-chain constructs were added for the co-transfections: (a) the commonality of high expression when λ light chains were paired with µ heavy chains to generate different IgMs; (b) the IgM concentrations in culture supernatants of Expi293 cells were determined 5 days post-transfection using ELISA. Error bars represent standard errors of the means. Data are representative of two independent experiments; (c) the fold difference when using λ light chains is summarized.