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. 2022 May 18;11(2):37. doi: 10.3390/antib11020037

Figure 2.

Figure 2

Antigen-specific mAb concentrations of isotypes IgG1, dIgA1, dIgA2, and IgM in culture supernatants of Expi293 cells were determined 5 days post-transfection using ELISA. Four different panels to test the preference for κ versus λ light chains with four different heavy chains were generated for (a) VRC01, an anti-HIV Env CD4 binding site mAb [7]; (b) Fm-6, an anti-SARS spike protein mAb [12]; (c) 33C6, an anti-HIV Env V3 loop mAb [13]; and (d) PGT121, a broadly neutralizing anti-HIV Env mAb targeting a complex glycan-dependent epitope [8]. Bars representing IgG1 (grey), dIgA1 (green), dIgA2 (blue), and IgM (black) are either open bars for the κ or solid bars for the λ light-chain versions. Error bars represent standard errors of the means. Data are representative of two independent experiments.