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. 2022 May 18;11(2):37. doi: 10.3390/antib11020037

Figure 4.

Figure 4

Expression of recombinant mAbs of different Ig classes without variable heavy and light regions. Antibody concentrations in Expi293 cell supernatants were determined 5 days post-transfection using ELISA: (a) recombinant mAb levels in culture supernatants when cotransfected with κ or λ light chains lacking variable regions; (b) concentrations of recombinant mAbs lacking variable regions in the presence or absence of a J-chain-expressing plasmid. In the absence of J chains, IgAs are expressed in multiple forms [16]. Bars representing IgG1 (grey), IgA1 (green), IgA2 (blue), and IgM (black) are either open bars for the κ or solid bars for the λ light-chain versions. The error bars represent the standard errors of the means. Data are representative of two independent experiments.