Figure 5. MMV652103 induces a sub-G1 peak and G1 cell cycle arrest in MCF7 breast cancer cells. (A) Representative cell cycle profiles of MCF7 cells exposed to vehicle or IC50 MMV652103 for 24 and 48 h as determined by staining cells with propidium iodide and measuring their DNA content by flow cytometry. The table shows the proportion of cells at each phase of the cell cycle expressed as a percentage of the total number of cells analyzed ± SEM pooled from three independent experiments. (B) Western blot analyses of protein harvested from cells treated as indicated and incubated with antibodies against cell cycle markers cyclin A and cyclin B1 and p38 was used as a loading control. Densitometry readings were obtained using ImageJ and protein expression levels are represented as a ratio of protein of interest/p38 normalized to the vehicle control sample. Blots are representative of at least three independent experiments.
