Table 1. Median Values of Binding Parameters from Three Sets of RNA–Ligand Interaction, Values for In Vitro-Selected, and Naturally Occurring RNA–Ligands from ref (50)^a.

	kon (M–1 s–1)	koff (s–1)	Kd (M)
RNA (in vitro-selected)–ligand (N = 13)50	8.1 × 104	6.3 × 10–2	4.3 × 10–7
RNA (naturally occurring)–ligand (N = 24)50	5.5 × 104	1.9 × 10–2	3.0 × 10–7
HIV-1 TAR–ligand (N = 48, used in this work)	3.8 × 104	7.9 × 10–2	5.0 × 10–6

^aAdapted with permission from ref (50). Copyright 2017/RNA Cold Spring Harbor Laboratory Press for the RNA Society/RNA.