Table 2.
Calculated potential doses (cfu) a laboratory worker would be exposed to when undertaking procedures on the bench and within a functioning biological safety cabinet
Procedure | Aerosol concentration (cfu/m3) | Exposure time (min) | Dose cfu—procedure on an open bench | Dose cfu—procedure within a BSC |
---|---|---|---|---|
Handshake (10 mL) 109 | 580 | 10 | 87 | 0.00087 |
Pipette mixing 107 (96-well plate) | 190 | 10 | 29 | 0.00029 |
0.1 mL serial dilution 107 (Eppendorf) | 55 | 30 | 25 | 0.00025 |
Eppendorf flip off | 8000 | 5 | 600 | 0.006 |
BSC, biological safety cabinet.