Figure 2.

Antitumor effect of I.T. vaccination is dependent on CD8⁺ T cells. Mice were treated with the PC7A nanovaccine on days 10, 17 and 24. One day before vaccine treatment, 250 µg of depletion antibodies against CD8⁺ T cells (A), CD4⁺ T cells (B) or NK cells (C) were injected I.P. every 3 days for a total of four times(n=5). CD8⁺ T cells but not CD4⁺ T cells or NK cells are important for tumor eradication after I.T. vaccination. I.T. PBS was included as negative control in the experiments. I.T., intratumoral.