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. 2022 May 11;12:887294. doi: 10.3389/fonc.2022.887294

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Copyright © 2022 Jiang, Zhang, Aleksandrovich, Ye, Wang, Chen, Gao, Wang, Yan, Yang, Lu, Liu, Zhang, Wu, Yao, Sun, Rong, Timofeevich, Mahmutovich, Zheng, Chen and Zhao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RRM2 knockdown inhibits GBM proliferation and induces cell cycle arrest both in vitro and in vivo. After transfecting U87 and U251 cells with RRM2 siRNA for 48h, (A) RRM2 mRNA expression was examined by qRT-PCR, (B) RRM2 protein expression was analyzed by western blot assay, (C) cells viability were assessed using MTT, (D, E) cells cycle distribution were analyzed using flow cytometry. (green: G0-G1, yellow: S, and blue: G2-M). (F) CDK1 protein expression was analyzed by western blot assay. (G) H&E images of brain sections of mice orthotopically xenografted with U251 cells transfected with LV-shNC and LV-shRRM2. Scale bar: 1000 μm. (H) IHC staining of CDK1 and RRM2 in consecutive brain sections of mice orthotopically xenografted with U251 cells transfected with LV-shNC and LV-shRRM2. Scale bar: 25 μm. (I) The survival time of nude mice orthotopically xenografted with U251 cells transfected with LV-shNC and LV-shRRM2. The data are presented as the mean ± SD (n=3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.