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. 2022 May 11;12:887294. doi: 10.3389/fonc.2022.887294

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Copyright © 2022 Jiang, Zhang, Aleksandrovich, Ye, Wang, Chen, Gao, Wang, Yan, Yang, Lu, Liu, Zhang, Wu, Yao, Sun, Rong, Timofeevich, Mahmutovich, Zheng, Chen and Zhao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PECT inhibits GBM proliferation, promotes cell cycle arrest, and increases autophagic flux by decreasing RRM2 expression in vitro. After U87 and U251 cells transfected with RRM2 overexpression plasmid or cultured with PECT (20 μM, 48 h), (A) cells viability were assessed using MTT, (B) RRM2 mRNA expression was examined by qRT-PCR, (C) RRM2 protein expression was analyzed by western blot assay, (D, E) cells cycle distribution were analyzed using flow cytometry. (green: G0-G1, yellow: S, and blue: G2-M). (F) CDK1, p62, LAMP2 and LC3B protein expression were analyzed by western blot assay. Vector and RRM2 represent negative control plasmid and RRM2 overexpression plasmid, respectively. The data are presented as the mean ± SD (n=3). NS: non-significant. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.