FIG. 2.

Alignment of the borders of seven copies of Abr1. (A) The source of each copy is indicated at the end of each line. The target site duplications and the TIRs are indicated by boxes. The alignment of target sites of all of the Abr1 copies shows a clear conservation in sequence (shaded bases). Primers flanking Abr1.1 in strain H97 and Abr1.2 in strain H39 were used to amplify genomic DNA of both strains H39 and H97. Sequences of the amplified fragments (B) allowed the identification of the target site (boxed sequences) in the strain lacking the corresponding fragments of Abr1. The TIRs are indicated by arrows.