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. 2022 May 30;20:75. doi: 10.1186/s12964-022-00881-9

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visithttp://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — The regulation mechanism of circPhf21a was determined by RNA-sequencing and RT-qPCR. A Heatmap of the differentially expressed genes, with | log2 (fold change) | > 2 and p < 0.001, between PMCs transfected with oe-NC and oe-circPhf21a plasmid. PMCs transfected with oe-NC are the control for the experiment. Red rectangles represent high expression, and green rectangles represent low expression. B Volcano plot of the differentially expressed mRNAs between PMCs transfected with oe-NC and oe-circPhf21a plasmids. The red plots represent upregulated genes, the black plots represent nonsignificant genes, and the blue plots represent downregulated genes. C–L The mRNA expression levels of the 10 most differentially expressed genes were detected by RT-qPCR. n = 3, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. M, N PMCs were incubated with negative control vector or TGF-β1 with or without oe-circPhf21a. After 48 h of incubation, mRNA and protein expression levels of Vegfa were detected by RT-qPCR or western blotting analysis. n = 3, *p < 0.05, ***p < 0.001