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. 2022 May 30;20:75. doi: 10.1186/s12964-022-00881-9

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visithttp://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 9 — Silence of circPhf21a alleviated the progression of OA in mouse model. A RT-qPCR analysis of circPhf21a expression in knee articular cartilage from OA mice in different groups (n = 3), **p < 0.01, ****p < 0.0001. B Schematic of the time course used for the DMM-induced in vivo osteoarthritis experiments. C H&E staining was performed to observe the cell morphology and tissue integrity in the articular cartilage tissues of the mouse knee in different groups. The figures located at the top show the extent of damage and the morphology of chondrocytes in the contact area of the weight-bearing region between the medial tibial plateau and the medial femoral condyle. Likewise, the middle and bottom figures show Safranin O and fast green staining of articular cartilage tissues from mice that underwent sham or DMM surgery. Safranin O and fast green staining demonstrated OA progression through the 8-week time course in the medial tibial plateau. Scale bar = 50 or 100 μm. D OARSI scores of the medial tibial plateau of different group mice (Sham, n = 6; DMM + sh-NC, n = 6; DMM + sh-circPhf21a, n = 6). ***p < 0.001, ****p < 0.0001. E Representative images for Col2a1 (green), Mmp13 (green) and Vegfa (green) immunofluorescent staining in cartilage tissues obtained from sham or DMM mouse knees (Sham, n = 6; DMM + sh-NC, n = 6; DMM + sh-circPhf21a, n = 6). Scale bar = 50 μm. The bar graphs show quantification (%) of the Col2a1, Mmp13 or Vegfa positive cells from total cell population per field in immunofluorescent sections