Table 1.
Primers used in the present study
| Primers | Sequences (5′–3′) | Target genes | PCR product size |
|---|---|---|---|
| EC ETT2a-F | ACGCTGATCGTGGGTATCCT | yqeG | 3118 bp |
| EC ETT2a-R | CTCACGATCTGTCAATGATGAG | yqeK | |
| EC ETT2b-F | GAGTCGAACTCGACATTCATC | yqeK | 3584 bp |
| EC ETT2b-R | CTGGGTTCGAGTTGTAGTGA | ygeH | |
| EC ETT2c-F | CTGATAACTGGTCTGTCTGGAC | ygeH | 3907 bp |
| EC ETT2c-R | TGCAGATGCACTCGTTTCATC | eprK | |
| EC ETT2d-F | ACTATCGCCAGTATCAACATCG | eprK | 3894 bp |
| EC ETT2d-R | CTCATACTGTATTGTCTCGGCAG | epaS | |
| EC ETT2e-F | TGTGAGTAGGGTTGGCAATC | epaS | 3310 bp |
| EC ETT2e-R | GATAGCAGAAGAGAGTGGCAG | epaO | |
| EC ETT2f-F | GAGCTGGACTCAATACTCCATC | epaO | 2872 bp |
| EC ETT2f-R | GCAGAACTTGACGACGTTTCTG | eivC | |
| EC ETT2 g-F | CCTGGCTATACGTTCATTTGC | eivC | 6321 bp |
| EC ETT2 g-R | AGGTCTGGTTGTGACATCGAC | ECs3735 | |
| EC ETT2 h-F | GTCGGATTTACGAATCATGAGA | ECs3735 | 2553 bp |
| EC ETT2 h-R | GGGATTGATATCTCAGCTCCAC | ECs3738 | |
| EC eicA-F | CAGAGCTGCATGGCATTACT | eicA | 369 bp |
| EC eicA-R | CAAGATAAGCAGTAGCCATCTC | eicA | |
| EC eipB-F | ACAGCCATTATGGATGCTGA | eipB | 610 bp |
| EC eipB-R | TCGTATCTTGATCGACACCA | eipB | |
| EC eipX-F | CAAGCAGTTCTACAGAGCAGAC | eipX | 738 bp |
| EC eipX-R | GATCCAGATAGCTACTCATAACTG | eipX | |
| EC eipD-F | GAGTTCTGCACCTGAAGTGAG | eipD | 557 bp |
| EC eipD-R | CTGAAGACTGACTCATACTGTC | eipD | |
| EC eilA-F | GTACCTATTGTGATTGTGACCG | eilA | 465 bp |
| EC eilA-R | CATAACAGCAGAATCCAGAGA | eilA | |
| EC eaeX-F | CGTTACTGTGACGGTTAATGG | eaeX | 478 bp |
| EC eaeX-R | GACGATTGCCAGTACCAATCAG | eaeX |