. Author manuscript; available in PMC: 2022 Oct 1.

Published in final edited form as: Nature. 2022 Mar 30;604(7905):362–370. doi: 10.1038/s41586-022-04544-0

Extended Data Table 2.

List of oligonucleotides.

PURPOSE	SEQUENCE
AnTaT VSG qPCR	ACAACCACGGAAAGTGACG CACTTTTTGTCGCCATAAGC
VSG2qPCR	AGCAGCCAAGAGGTAACAGC CAACTGCAGCTTGCAAGGAA
VSG117 qPCR	AAGCGACAACAGATAAATGC CTTTGCAAGCATTATTTTCC
18S qPCR	ACGGAAT GGCACCACAAGAC GTCCGTTGACGGAATCAACC
16-mer mutagenesis	TTTGTTATACAAAACTTTTCAAAACCAGCCGAGATTTTGTG TTTGAAAAGTTTTGTATAACAAAAGTTTTCAAGTAGCAAGG
PAT adaptor	5’-Ph-CCAGTGAGCAGAGTGACGAGGACTCGAGCTCAAGC- 3ddA-3’
PAT Rev 1	GCTTGAGCTCGAGTCCTCG
PAT Rev 2	CGTCACTCTGCTCACTGG
PAT rev A0	GAGGACTCGAGCTCAAGCGCGTGTTAAAATATATCAG
PAT AnTaT 1	AATCCCCGAATTGTAAATGG
PAT AnTaT 2	TTTCTGCCGCATTTGTGG
PAT VSG117 1	AAGCGACAACAGATAAATGC
PAT VSG117 2	ATTCGCCCTCAGTGCTGC
AnTaT VSG RNase H A	TACTCGTCGTTGGCTGCTTG
AnTaT VSG RNase H B	TATTTTACTGCATAGGGCGT
AnTaT VSG RNase H C	GCGTGTTAAAATATATCAGA
Spliced leader RNase H	CAATATAGTACAGAAACTGT
β-tubulin RNase H	TACGGAGTCCATTGTACCTG
Oligo d(T)	TTTTTTTTTTTTTTTTTT
VSG2 RNase H	TCCGGCTGTTTCGTTTCT
Dot blot oligo A	ACTAGCTTAACTACGACCTCCTGAG
Dot blot oligo m⁶A	ACTAGCTTAACT-m⁶A-CGACCTCCTGAG
Dot blot oligo m¹A	ACTAGCTTAACT-m¹A-CGACCTCCTGAG GGAGAAAGAATAGTAACCCTTTCATCAAAGAAAATAGTCGAA
VSG8 cloning	GCTTATGCGAACAGCGAGCACAACC GAGGGGGGAAATTTGAGGGGGGAAAGGGCTGCAGGAATTC TTAAAAAAGCAAGGCCACAAATGC
VSG8qPCR	ATGGAAAGGAACCAACAACG CCCTCCTTTGTCTTATCTTTGC GGAGTTTGGTATAATCCGTTCG
CAF1qPCR	GCGTGGATTTGAAGTTACCG
PAT VSG2 1	AAGGTAGCAGATGAGACTGC
PAT VSG2 2	TTAGCAAGACCCCTCTTTGG
VSG RNase H northern	ACGCCCTATGCAGTAAAATA
VSG motif probe	GCGTGTTAAAATATATC