Extended Data Table 3.
Statistical parameters of time course experiments.
| Figure | Measure | Experimental approach | Curve fit | Lag phase (XO) (min) | Decay constant (K) (min−1) | Half-life (min) | R2 |
|---|---|---|---|---|---|---|---|
|
| |||||||
| 2b | mRNA | Transcription inhibition (ActD) | Biphasic | 60.0 | 0.019 | 36.9 | 0.91 |
| 2b | poly(A) | Transcription inhibition (ActD) | Biphasic | 42.9 | 0.015 | 45.4 | 0.95 |
| 2b | m6A | Transcription inhibition (ActD) | Exponential | NA | 0.019 | 37.4 | 0.93 |
| 2d | mRNA | Differentiation | Biphasic | 53.5 | 0.012 | 58.7 | 0.88 |
| 2d | poly(A) | Differentiation | Exponential | NA | 0.031 | 22.7 | 0.93 |
| 5d | mRNA | Transcription inhibition (ActD) | Exponential | NA | 0.019 | 36.8 | 0.95 |
| 5b | poly(A) | Transcription inhibition (ActD) | Exponential | NA | 0.074 | 9.3 | 0.98 |
| S4 | mRNA | Transcription inhibition (ActD) | Biphasic | 32.7 | 0.009 | 76.3 | 0.85 |
| S4 | poly(A) | Transcription inhibition (ActD) | Biphasic | 27.8 | 0.006 | 114.5 | 0.91 |
| S4 | m6A | Transcription inhibition (ActD) | Exponential | NA | 0.026 | 26.2 | 0.97 |
Curves were fitted to the decay of VSG mRNA (pink), length of poly(A)-tail (dark blue) and m6A levels (light blue). Curves in which the measured variable decayed from T=0hr were called “One phase”. Those in which the measured variable decayed only after an initial constant period were called “Biphasic”.