Fig. 1.

Anti-Toxoplasma gondii immunoglobulins detected by conventional ELISA (IgG) and sporozoite-specific embryogenesis-related protein (TgERP) ELISA (IgG and IgA) to discriminate controls (■) from patients (□) in the different age groups for the individuals enrolled in the study. Data are expressed as scattering distribution of optical density (OD) for all immunoglobulins. ROC curve analysis was used to establish the best cut-off edge to segregate immunoglobulin reactivity in the groups. The cut-off edges and the performance indices (Se, Sp, AUC and LR+) are provided. (a) IgG reactivity assessed in sera samples for TgERP ELISA. (b) IgA reactivity assessed in saliva samples for TgERP ELISA. The number of individuals in each group is denoted within parentheses. The subjects included in control groups were those that tested negative for conventional ELISA against T. gondii (IgG and/or IgM) and varied according to age group: 0–7 years (n = 104 subjects), 8–14 years (n = 99), 15–21 years (n = 15), and 22–28 years (n = 15).