Table 1.
Direction | Sequence (5′3′) | Site of the primers | Product size (bp) | |
---|---|---|---|---|
First round | Forward | AGAAAATGGTTGGATGTGGTGAG | 744–766 | 1201 |
Reverse | GCTCCTGTCCTGGGTTGTCTGAT | 1923–1945 | ||
Second round | Forward | GCTATGCCATGGGAGTAGGAGTGG | 1108–1131 | 593 |
Reverse | CCTCGGCCTCTCGCACCTAGT | 1681–1701 |