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. 2022 May 16;9:821272. doi: 10.3389/fnut.2022.821272

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Copyright © 2022 Meng, Deng, Xiao, Arowolo, Liu, Chen, Deng, He and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of heat stress on the splenic transcriptome of 42-day-old broilers (d 14 of heat treatment). (A) Numbers of heat stress-induced differentially expressed splenic genes (P < 0.05 and fold change ≥ 2; n = 4). (B) GO terms enrichment of splenic gene expression for up genes in broilers (P < 0.05). (C) GO terms enrichment of splenic gene expression for down genes in broilers (P < 0.05). (D) KEGG terms enrichment of splenic gene expression for down genes in broilers (P < 0.05). (E) The heat map of DEG genes in broilers. (F) Validation of the gene expression profile by real-time PCR. Asterisk means significant difference with NT group (*P < 0.05). RSV, resveratrol; NT, normal temperature; HT, heat stress.