Fig. 11.

(a) Time-lapse images of background-subtracted and normalized $i_{\mathrm{AA}}$ and (b) FRET efficiency of one growth cone from a neuron expressing TSMod. (c) Pixels with FRET efficiency $\ge 0.25$ (red) and those with FRET efficiency $<0.25$ (blue) are segregated to highlight the spatial differences in FRET efficiency. (d) Mean FRET efficiency of the growth cone as a function of time. (e) Area fraction of pixels with high ($\ge 0.25$) and low ($<0.25$) FRET efficiency. The field of view is $24\times 24\mu {\mathrm{m}}^2$ in each image.