Fig. 2.

Raw images of growth cones expressing VinTS. The three images are the output of three imaging channels: donor (${\mathrm{mTFP}}_1$) excitation and donor emission, $I_{\mathrm{DD}}$ (cyan); acceptor (mVenus) excitation and acceptor emission, $I_{\mathrm{AA}}$ (yellow); FRET channel, donor excitation, and acceptor emission, $I_{\mathrm{DA}}$ (red). The original field of view is $128\times 128\mu \mathrm{m}$ (EM-CCD). Growth cones regions (red boxes) were selected for processing to calculate FRET efficiency. Under each original image are the corresponding selected growth cone areas. The field of view in the cropped regions with the growth cones is $24\times 24\mu {\mathrm{m}}^2$.