Figure 5.

Nanobody-IgG1 constructs are able to induce Fc-mediated effector functions. Neutralization capacity of IgG1 fusion constructs compared to monovalent or bivalent versions of (A) J3 (B) 1F10 and (C) 2E7 and (D) J3-2E7 and (E) J3-1F10. (F) Neutralization capacity of J3-IgG1 compared to bispecific J3-2E7-IgG1 and J3-1F10-IgG1. Friedman matched comparison was used to compare neutralization IC₅₀ (µM) between three groups. Wilcoxon matched-pairs signed rank tests was used to compare neutralization IC₅₀ (µM) between two groups. Significance is indicated as *p <0.05, **p <0.005, ***p <0.0005 and not significant (ns). Data shown are the average of three independent experiments.