Skip to main content
. 2022 May 30;11:e72359. doi: 10.7554/eLife.72359

Figure 1. Dnmt3a-HET mice become overweight and develop obesity.

(A) Weight of male Dnmt3a-HET (HET, n=14) and WT (n=12) littermates on regular chow. * p=0.03, ***<p = 0.001 as determined by two-way ANOVA. (B) Distribution of weight from HET and WT mice females and males at 1 year of age. Dashed lines indicate 3 standard deviations (SD) above the mean of the WT. (C) Representative display of D3A-HET and WT mice at 1 year Left: D3A-HET and WT mice at 1 year. Right: dual energy X-ray absorptiometry (DEXA) analysis of 6-month-old WT and HET mice. Top: Representative image displaying lean (brown color) and fat (grey) tissues. (D) Quantification of fat and lean mass (n=10–14) from DEXA. Statistical analysis 1way ANOVA. (E) Representative H&E staining of gonadal white adipose tissue and BAT from 1-year-old mice. (F) Morphometric analysis of gWAT and BAT adipocyte diameter. ***p<0.001 by 1way ANOVA. (G) Representative staining of liver tissue from 1-year-old WT and 3A-HET littermates. Top: H&E staining. Bottom: Oil Red O lysochrome diazo dye. (H) Quantification of total red droplets from Oil Red O-stained livers (n=3 per group). Statistical analysis one-way ANOVA.

Figure 1—source data 1. List of cluster marker genes.

Figure 1.

Figure 1—figure supplement 1. Dnmt3a-HET mice recapitulate TBRS phenotypes.

Figure 1—figure supplement 1.

(A) Body weight gain of female Dnmt3a-HET (HET, n=12) compared to their WT (n=14) littermates on regular chow diet starting at 2 months of age. **p=0.02,***, ****p<0.001 by two-way ANOVA. (B) Mouse length nose to rump. p<0.05 by two-tailed unpaired Student’s t test. (C) Displayed percentage of fat from total body weight as measured from dual energy X-ray absorptiometry (DEXA). Fat composition analysis was done on 6 month and 1 year old WT and 3A-HET littermates. **p<0.01, ***p<0.001 by two-way ANOVA. (D) Fat mass of inguinal white adipose tissue (iWAT), gonadal white adipose tissue (gWAT) and brown adipose tissue (BAT) of WT (n=4–5) and 3A-HET (n=5) mice at 1 year. (E) F4\80 quantification in inguinal WAT in 1-year-old HET mice. Adipose tissue was dissected and F4\80+, Cd45 +macrophage cells were quantified by flow cytometry. (F) Adipocyte area as measured in histological sections of inguinal WAT.