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Primers used for detection of E. coli O157 genes
| Primer | Sequence | Target gene | Amplicon size (bp) | Reference |
|---|---|---|---|---|
| rfbE_F | CTACAGGTGAAGGTGGAATGG | rfbEO157 | 327 | Wang et al. [30] |
| rfbE_R | ATTCCTCTCTTTCCTCTGCGG | |||
| Stx1_F | ATAAATCGCCATTCGTTGACTAC | stx1 | 180 | Paton & Paton [31] |
| Stx1_R | AGAACGCCCACTGAGATCATC | |||
| Stx2_F | GGCACTGTCTGAAACTGCTCC |
stx2 (including stx2 variants) |
255 | |
| Stx2_R | TCGCCAGTTATCTGACATTCTG |
