Table 1.
Molecular characterization of neonatal septicaemic Escherichia coli isolated from three different hospitals
| Isolate no. | Centre, year of isolation | Phylo-group | Virulence factors | Serotypes | Antibiogram | Extended spectrum β-lactamses (ESBLs) Metallo-β-lactamases (MBLs) |
|---|---|---|---|---|---|---|
| EC1 | SSKM, 2007 | A | traT, hlyA, PapC, IroNEC, iha | O15 | CTX-S, AK-S, CIP-S, SXT-S, IMP-S | ESBL-negative |
| EC2 | Delhi, 2008 | A | iucC, traT, iha | — | CTX-S, AK-R, CIP-R, SXT-S, IMP-S | ESBL-negative |
| EC3 | Delhi, 2008 | C | iucC, traT | — | CTX-R, AK-R, CIP-R, SXT-S, IMP-S | OXA-1, CTX-M-15 |
| EC4 | Delhi, 2008 | A | iucC, iroNE.coli, traT | — | CTX-R, AK-S, CIP-R, SXT-R, IMP-S | OXA-1, CTX-M-15 |
| EC5 | Delhi, 2009 | A | iucC, iha | O2a | CTX-S, AK-S, CIP-S, SXT-S, IMP-S | CTX-M-15 |
| EC6 | Sheuri, 2009 | C | Nil | — | CTX-R, AK-R, CIP-R, SXT-R, IMP-S | TEM, OXA-1, CTX-M-15 |
| EC7 | Sheuri, 2009 | C | traT | — | CTX-R, AK-R, CIP-R, SXT-R, IMP-R | CTX-M-15, NDM-1 |
| EC8 | SSKM, 2011 | C | traT | — | CTX-R, AK-R, CIP-R, SXT-R, IMP-R | CTX-M-15, NDM-1 |
| EC9 | SSKM, 2011 | C | hlyA, traT, cnf | — | CTX-R, AK-R, CIP-R, SXT-R, IMP-R | TEM-1, CTX-M-15, NDM-1 |
| EC10 | SSKM, 2008 | A | iucC | — | CTX-R, AK-S, CIP-R, SXT-S, IMP-S | ESBL-negative |
| EC11 | SSKM, 2007 | B1 | iucC | — | CTX-R, AK-R, CIP-R, SXT-R, IMP-S | OXA-1, CTX-M-15 |
| EC12 | Delhi, 2008 | B1 | iucC | — | CTX-R, AK-S, CIP-R, SXT-R, IMP-S | OXA-1, CTX-M-15 |
| EC13 | Delhi, 2008 | F | papC | O1 | CTX-R, AK-S, CIP-R, SXT-R, IMP-S | OXA-1, CTX-M-15 |
| EC14 | Delhi, 2008 | A | iucC, hlyA, papC, iha | O2a | CTX-S, AK-S, CIP-S, SXT-S, IMP-S | ESBL-negative |
| EC15 | Delhi, 2008 | B1 | Nil | O4 | CTX-R, AK-S, CIP-R, SXT-R, IMP-S | OXA-1, CTX-M-15 |
| EC16 | Delhi, 2008 | B1 | iucC | — | CTX-R, AK-S, CIP-R, SXT-R, IMP-S | OXA-1, CTX-M-15 |
| EC17 | Delhi, 2009 | B1 | Nil | — | CTX-S, AK-S, CIP-R, SXT-R, IMP-S | TEM, CTX-M-15 |
| EC18 | Delhi, 2009 | E | iucC, traT | — | CTX-R, AK-R, CIP-R, SXT-R, IMP-S | TEM, CTXM-15 |
| EC19 | Delhi, 2009 | B1 | traT | — | CTX-S, AK-S, CIP-S, SXT-R, IMP-S | CTX-M-15 |
| EC20 | Delhi, 2009 | B1 | traT | — | CTX-R, AK-S, CIP-R, SXT-R, IMP-S | TEM, OXA-1, CTX-M-15 |
| EC21 | Delhi, 2009 | E | iucC, papC | O18 | CTX-R, AK-S, CIP-R, SXT-R, IMP-S | CTX-M-15 |
| EC22 | SSKM, 2011 | B1 | traT | — | CTX-S, AK-S, CIP-S, SXT-R, IMP-S | SHV, TEM, CTX-M |
| EC23 | Delhi, 2009 | B1 | iucC, usp | O25a | CTX-R, AK-S, CIP-R, SXT-R, IMP-S | CTX-M-15 |
| EC24 | SSKM, 2007 | B2 |
hlyA, sfa, papC, iroNE.coli, cnf, traT, usp |
O6a | CTX-S, AK-S, CIP-S, SXT-S, IMP-S | ESBL-negative |
| EC25 | Delhi, 2008 | B2 | iucC, papC, traT | — | CTX-R, AK-S, CIP-R, SXT-S, IMP-S | OXA-1, CTX-M-15 |
| EC26 | Delhi, 2008 | B2 | iucC, papC, traT, usp | O25b | CTX-R, AK-S, CIP-R, SXT-S, IMP-S | OXA-1, CTX-M-15 |
| EC27 | Delhi, 2008 | B2 | iucC, hlyA, papC, iroNE.coli, sfa, iha, usp | — | CTX-S, AK-S, CIP-S, SXT-S, IMP-S | ESBL-negative |
| EC28 | Delhi, 2008 | B2 | iucC, papC, traT, usp | O25b | CTX-R, AK-S, CIP-R, SXT-S, IMP-S | OXA-1, CTX-M-15 |
| EC29 | Delhi, 2008 | B2 | iucC, hlyA, PapC, iroNE.coli, cnf, traT, usp | O25b | CTX-R, AK-S, CIP-R, SXT-R, IMP-S | OXA-1, CTX-M-15 |
| EC30 | Delhi, 2008 | B2 | iucC, papC, iroNE.coli, usp | O25b | CTX-R, AK-R, CIP-R, SXT-S, IMP-S | OXA-1, CTX-M-15 |
| EC31 | SSKM, 2009 | B2 | iucC, papC | O25b | CTX-R, AK-S, CIP-S, SXT-R, IMP-R | CTX-M-15, NDM-1 |
| EC32 | Delhi, 2009 | B2 | iucC, papC, traT, usp | O25b | CTX-R, AK-R, CIP-R, SXT-S, IMP-S | CTX-M-15 |
| EC33 | Delhi, 2009 | B2 | iucC, hlyA, sfa, papC, iroNE.coli, traT, iha, usp | O25b | CTX-S, AK-R, CIP-S, SXT-R, IMP-S | TEM, CTX-M-15 |
| EC34 | SSKM, 2008 | B2 | iucC, traT, hlyA, cnf, iha, usp | O75 | CTX-R, AK-S, CIP-S, SXT-R, IMP-S | TEM, CTX-M-15 |
| EC35 | SSKM, 2009 | B2 | papC, iroNE.coli, traT, hlyA, sfa, cnf | O6a | CTX-R, AK-R, CIP-R, SXT-R, IMP-S | CTX-M-15 |
| EC36 | SSKM, 2010 | B2 | iucC, hlyA, papC, traT, usp | — | CTX-R, AK-R, CIP-R, SXT-R, IMP-S | SHV, TEM, CTX-M-15 |
| EC37 | SSKM, 2011 | B2 | iucC, papC, traT, iha, usp | O25b | CTX-R, AK-R, CIP-R, SXT-R, IMP-S | CTX-M-15 |
| EC38 | SSKM, 2011 | B2 | iucC, papC, traT, iroNE.coli, usp | — | CTX-S, AK-S, CIP-S, SXT-S, IMP-S | ESBL-negative |
| EC39 | SSKM, 2008 | B2 | iucC, papC, traT, usp | O75 | CTX-S, AK-S, CIP-S, SXT-R, IMP-S | SHV, TEM |
| EC40 | SSKM, 2008 | B2 | iucC, papC, traT, hlyA, usp | O75 | CTX-S, AK-S, CIP-S, SXT-R, IMP-S | SHV, TEM, CTX-M-15 |
| EC41 | SSKM, 2008 | B2 | iucC, papC, traT, usp | O75 | CTX-S, AK-S, CIP-S, SXT-R, IMP-S | ESBL-negative |
| EC42 | SSKM, 2008 | B2 | iucC, papC, traT, iha | O25b | CTX-R, AK-R, CIP-R, SXT-R, IMP-R | TEM-1, CTX-M-15, NDM-1 |
| EC43 | SSKM, 2009 | E | iucC, traT | — | CTX-R, AK-R, CIP-R, SXT-S, IMP-R | TEM-1, OXA-1, NDM-1 |
| EC44 | SSKM, 2010 | B2 | iucC, papC, traT, usp | O75 | CTX-S, AK-S, CIP-S, SXT-R, IMP-S | TEM, CTX-M-15 |
| EC45 | SSKM, 2010 | B2 | iucC, iroNE.coli, traT, usp | O25b | CTX-R, AK-R, CIP-R, SXT-R, IMP-R | TEM-1, CTX-M-15, NDM-1 |
| EC46 | SSKM, 2007 | D | Nil | — | CTX-R, AK-S, CIP-R, SXT-S, IMP-S | OXA-1, CTX-M-15 |
| EC47 | Delhi, 2008 | D | iucC, papC, traT, hlyA, iha | — | CTX-R, AK-R, CIP-R, SXT-R, IMP-S | OXA-1, CTX-M-15 |
| EC48 | Delhi, 2008 | D | traT | O15 | CTX-S, AK-S, CIP-S, SXT-R, IMP-S | ESBL-negative |
| EC49 | Delhi, 2009 | E | traT | — | CTX-R, AK-S, CIP-R, SXT-R, IMP-S | CTX-M-15 |
| EC50 | Delhi, 2009 | D | iucC, traT, iha | O157 | CTX-R, AK-S, CIP-R, SXT-R, IMP-S | CTX-M-15 |
| EC51 | Delhi, 2009 | B2 | iucC, traT | — | CTX-R, AK-R, CIP-R, SXT-R, IMP-S | TEM, CTX-M-15 |
| EC52 | Delhi, 2009 | D | iucC, traT | — | CTX-R, AK-R, CIP-R, SXT-R, IMP-S | TEM, CTX-M-15 |
| EC53 | Sheuri, 2009 | E | iucC, traT | — | CTX-R, AK-R, CIP-R, SXT-R, IMP-S | TEM |
| EC54 | Sheuri, 2009 | D | iucC, traT | — | CTX-R, AK-R, CIP-R, SXT-R, IMP-S | TEM, CTX-M-15 |
| EC55 | Sheuri, 2009 | E | iucC, traT | — | CTX-R, AK-R, CIP-R, SXT-R, IMP-S | TEM, CTX-M-15 |
| EC56 | Sheuri, 2009 | D | iucC, traT | — | CTX-R, AK-R, CIP-R, SXT-R, IMP-S | TEM, CTX-M-15 |
| EC57 | Sheuri, 2009 | E | iucC, traT | — | CTX-R, AK-R, CIP-R, SXT-R, IMP-S | CTX-M-15 |
| EC58 | Sheuri, 2009 | D | iucC, traT | — | CTX-R, AK-R, CIP-R, SXT-R, IMP-S | TEM, OXA-1 |
| EC59 | Sheuri, 2009 | E | iucC, traT | — | CTX-R, AK-R, CIP-R, SXT-R, IMP-S | CTX-M-15 |
| EC60 | Sheuri, 2010 | F | papC, traT | O1 | CTX-R, AK-R, CIP-R, SXT-R, IMP-R | OXA-1, TEM-1, CTX-M-15, NDM-1 |
| EC61 | SSKM, 2011 | D | papC,hlyA,traT,cnf | O25a | CTX-S, AK-S, CIP-S, SXT-R, IMP-S | CTX-M-15 |
| EC62 | SSKM, 2011 | D | Nil | O12 | CTX-R, AK-S, CIP-S, SXT-R, IMP-S | CTX-M-15 |
| EC63* | SSKM, 2011 | D | iucC, traT | — | CTX-S, AK-S, CIP-S, SXT-R, IMP-S | CTX-M-15 |
| EC64 | SSKM, 2008 | D | iucC, papC, hlyA | — | CTX-R, AK-S, CIP-R, SXT-R, IMP-S | TEM, OXA-1, CTX-M-15 |
| EC65* | SSKM, 2008 | E | iucC, hlyA, papC, cnf, traT, iha | O17 | CTX-R, AK-R, CIP-R, SXT-R, IMP-R | TEM, OXA-1, CTX-M |
| EC66 | SSKM, 2008 | D | Nil | — | CTX-R, AK-S, CIP-R, SXT-R, IMP-S | SHV, TEM, OXA-1, CTX-M-15 |
| EC67 | SSKM, 2011 | A | Nil | — | CTX-R, AK-R, CIP-R, SXT-R, IMP-R | TEM-1, OXA-1, CTX-M-15, NDM-1 |
AK, Amikacin; CIP, ciprofloxacin; CTX, cefotaxime; SXT, trimethoprim-sulfamethoxazole; IMP, imipenem.
Nil, No virulence genes detected; -, targeted O serotypes not present.
*
Clonal group A.