Table 1.
Flu A(H3) and Flu A(H1) primers and thermal profile used in viral genome amplification
| Detection method | Gene | Primer sequences 5′–3′ | Location | Thermal profile |
|---|---|---|---|---|
| Flu A(H3N2) | ||||
| PCR | HA-1 | H3-forward (H3f) | 250–273 | 95°C for 10 min (×1) |
| CCTTGATGGAGAAAACTGCACAC | 94°C for 45 s, 52°C for 30 s | |||
| H3-reverse (H3r) | 567–588 | 72°C for 30 s (×40) | ||
| TGTTTGGCATAGTCACGTTCA | 567–588 | 72°C for 5 min (×1) | ||
| Flu A(H1N1) | ||||
| PCR | HA-1 | H1- forward (H1f) | 303–327 | 95°C for 5 min (×1) |
| GAATCATGGTCCTACATTGTAGAAA | 95°C for 1 min, 55°C 1 min | |||
| H1-reverse (H1r) | 1092–1117 | 72°C for 1 min (×25) | ||
| ATCDATTCCAGTCCATCCCCCTTCAAT | 72°C for 7 min (×1) | |||
| Flu A/B multiplex real-time PCR | ||||
| FluA | M | GAGTCTTCTAACMGAGGTCGAAACGTA | 13 | FluAv_For |
| GGGCACGGTGAGCGTRAA | 207 | FluAv_Rev | ||
| FAM-TCCTGTCACCTCTGAC-MGB | FluAv_TM | |||
| FluB | M | TAGCAGAAGGCCATGAAAGCT | 317 | FluBv_For |
| CGTTCCTAGTTTTACTTGCATTGAAT | 411 | FluBv_Rev | ||
| JOE-TGTCTCATGGTCATGTACCT-MGBNFQ | FluBv_TM | |||