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Sensitivity of the LAMP assay using primer set 2 on serially diluted DNA of Leishmania tropica 101/65 at 63°C for 85 min. Lane 1, 3·6 ng; 2, 360 pg; 3, 36 pg; 4, 3·6 pg; 5, 360 fg; 6, 36 fg; 7, 3·6 fg; 8, negative control; 9, positive control (L. infantum); M, 100 bp DNA ladder.
