Table 1.

Nucleotide sequences of LAMP primers (sets 1 and 2) designed for detection of Leishmania promastigotes

Assay	Primer type	Primer length	Sequence	Amplicon size	Target gene
LAMP set 1	FIP (F1c-F2)	39	5′-CACCGGACGCACTAAACCCC-GTGCTTTCCCATCGCAACT-3′	198	FR796 423 Leishmania major strain Friedlin complete genome, chromosome 27 from nt 990 604–990 773
	BIP (B1-B2c)	39	5′-TCCGGCCGTAACGCCTTTTC-CAGTACGTTCTCCCCCGAA-3′
	F3	20	5′-ATTATGGAGCTGTGCGACAA-3′
	B3	20	5′-AGAATTTCACCTCTGACGCC-3′
LAMP set 2	FIP (F1c-F2)	41	5′-TGGGTAAAACCCGCCGATGAG-GCTGCCCAGTAGAATTCAGA-3′	190	FR796 423 Leishmania major strain Friedlin complete genome, chromosome 27 from nt 990 305 to 990 492
	BIP (B1-B2c)	42	5′-GGTCGAATTCTTCTCTGCGGGA-CATCACAGACCTGCTGTTGC-3′
	F3	19	5′-CGTCAACGGACGAGATCCA-3′
	B3	19	5′-CGCCCAGAACATTGAGGAG-3′