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. 2013 Dec 5;142(10):2096–2104. doi: 10.1017/S0950268813003014

Table 1.

Norovirus genogroup I (GI) and genogroup II (GII) concentrations and genotypes detected in outbreak samples

Outbreak Type Sampling point Lab ID Real-time RT-qPCR Genotypes (number of clones) Comment
GI GII GI GII
February 2010 Oyster Restaurant A REST79 <LOQ 2·35 × 103 n.t. II.3 (5) Oyster consumed at restaurant associated with gastroenteritis illness
Oyster Restaurant B REST80 <LOD 2·04 × 103 I.4 (5) II.3 (2), II.4 (1), II.13 (2) Oyster consumed at restaurant associated with gastroenteritis illness
Oyster Harvest area 1, site 1 HAR69 1·95 × 102 2·89 × 103 I.4 (4) II.3 (5), II.13 (2) Oysters harvested after outbreak occurred
Oyster Harvest area 1, site 2 HAR70 <LOD 1·92 × 103 I.4 (4) II.12 (3), II.13 (2) Oysters harvested after outbreak occurred
Stool* Patient STOOL * * * II.13 Sequence data obtained by direct sequencing
January 2012 Oyster Restaurant C REST10 <LOD 2·38 × 103 I.1 (5), I.4 (14) II.1 (5), II.3 (6), II.6 (2), II.4 (9) Oyster consumed at restaurant associated with gastroenteritis illness
Oyster Harvest area 2 HAR13 1·79 × 103 4·00 × 103 I.NA (2), I.2 (3), I.11 (2) II.1 (1), II.6 (2), II.7 (2) Oysters harvested after outbreak occurred
Stool Patient 2937 3·46 × 105 4·85 × 108 I.4 (5) II.1 (5) Cloning of the nested RT-PCR products was performed prior to sequencing
Stool Patient 2915 <LOD 9·81 × 108 I.1 (4), I.6 (1) II.1 (4)
Stool Patient 2906 1·32 × 108 2·21 × 108 I.4 (5) II.1 (4), II.7 (1)
Stool Patient 2761 2·54 × 103 5·66 × 109 I.2 (1), I.4 (2), I.6 (2) II.1 (5)

n.t., Not typed as nested RT-PCR amplification was unsuccessful.

*

Sample tested by National Virus Reference Laboratory in Dublin.

Real-time RT-qPCR results are expressed in genome copies/g faeces for stool samples and in genome copies/g digestive tissue for oyster samples.