Table 1.
Outbreak | Type | Sampling point | Lab ID | Real-time RT-qPCR | Genotypes (number of clones) | Comment | ||
---|---|---|---|---|---|---|---|---|
GI† | GII † | GI | GII | |||||
February 2010 | Oyster | Restaurant A | REST79 | <LOQ | 2·35 × 103 | n.t. | II.3 (5) | Oyster consumed at restaurant associated with gastroenteritis illness |
Oyster | Restaurant B | REST80 | <LOD | 2·04 × 103 | I.4 (5) | II.3 (2), II.4 (1), II.13 (2) | Oyster consumed at restaurant associated with gastroenteritis illness | |
Oyster | Harvest area 1, site 1 | HAR69 | 1·95 × 102 | 2·89 × 103 | I.4 (4) | II.3 (5), II.13 (2) | Oysters harvested after outbreak occurred | |
Oyster | Harvest area 1, site 2 | HAR70 | <LOD | 1·92 × 103 | I.4 (4) | II.12 (3), II.13 (2) | Oysters harvested after outbreak occurred | |
Stool* | Patient | STOOL | –* | –* | –* | II.13 | Sequence data obtained by direct sequencing | |
January 2012 | Oyster | Restaurant C | REST10 | <LOD | 2·38 × 103 | I.1 (5), I.4 (14) | II.1 (5), II.3 (6), II.6 (2), II.4 (9) | Oyster consumed at restaurant associated with gastroenteritis illness |
Oyster | Harvest area 2 | HAR13 | 1·79 × 103 | 4·00 × 103 | I.NA (2), I.2 (3), I.11 (2) | II.1 (1), II.6 (2), II.7 (2) | Oysters harvested after outbreak occurred | |
Stool | Patient | 2937 | 3·46 × 105 | 4·85 × 108 | I.4 (5) | II.1 (5) | Cloning of the nested RT-PCR products was performed prior to sequencing | |
Stool | Patient | 2915 | <LOD | 9·81 × 108 | I.1 (4), I.6 (1) | II.1 (4) | ||
Stool | Patient | 2906 | 1·32 × 108 | 2·21 × 108 | I.4 (5) | II.1 (4), II.7 (1) | ||
Stool | Patient | 2761 | 2·54 × 103 | 5·66 × 109 | I.2 (1), I.4 (2), I.6 (2) | II.1 (5) |
n.t., Not typed as nested RT-PCR amplification was unsuccessful.
Sample tested by National Virus Reference Laboratory in Dublin.
Real-time RT-qPCR results are expressed in genome copies/g faeces for stool samples and in genome copies/g digestive tissue for oyster samples.