Table 3.
The diagnostic sensitivity and specificity of the Seeplex® Diarrhea ACE Detection multiplex PCR system (Seeplex system) relative to the routine diagnostic testing algorithm for diagnosis of IID. The Seeplex system was compared to the routine diagnostic testing algorithm as the gold standard for enteric pathogens common to both diagnostic tests
| Pathogen | No. specimens tested | Concordant | Discordant | Sensitivity (%) | Specificity (%) | ||
|---|---|---|---|---|---|---|---|
| +/+ | −/− | +/− | −/+ | ||||
| Salmonella spp. | 201 | 16 | 182 | 0 | 3 | 84·21 | 100·00 |
| Shigella spp. | 201 | 4 | 194 | 3 | 0 | 100·00 | 98·48 |
| Campylobacter spp. | 201 | 16 | 184 | 2 | 0 | 100·00 | 98·92 |
| Escherichia coli O157 | 201 | 2 | 199 | 0 | 0 | 100·00 | 100·00 |
| Clostridium difficile | 63 | 5 | 53 | 0 | 5 | 50·00 | 100·00 |
| Vibrio spp. | 5 | 1 | 4 | 0 | 0 | 100·00 | 100·00 |
| Adenovirus | 64 | 11 | 51 | 2 | 0 | 100·00 | 96·23 |
| Rotavirus | 64 | 7 | 56 | 0 | 1 | 87·50 | 100·00 |
| Norovirus | 14 | 12 | 2 | 0 | 0 | 100·00 | 100·00 |
+/ + , Positive by the Seeplex system and routine diagnostic testing algorithm.
−/−, Negative by the Seeplex system and routine diagnostic testing algorithm.
+/−, Positive by the Seeplex system and negative by the routine diagnostic testing algorithm.
−/ + , Negative by the Seeplex system and positive by the routine diagnostic testing algorithm.